USP28 Rabbit Polyclonal Antibody
cat.: ER1803-52
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 122 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human USP28 aa 550-700. |
| Positive control: | K-562 (Human chronic myelogenous leukemia cell) cell lysate, Daudi (Human Burkitt's lymphoma cell) cell lysate, HaCaT (Human immortalized keratinocyte) cell lysate. |
| Subcellular location: | Nucleus, nucleoplasm. |
| Recommended Dilutions:
WB FC |
1:5,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q96RU2 Human |
| Alternative names: | Deubiquitinating enzyme 28 KIAA1515 Ubiquitin carboxyl terminal hydrolase 28 Ubiquitin carboxyl-terminal hydrolase 28 ubiquitin carboxyl-terminal hydrolase 28 variant 1 Ubiquitin specific peptidase 28 Ubiquitin specific processing protease 28 Ubiquitin specific protease 28 Ubiquitin thioesterase 28 Ubiquitin thiolesterase 28 Ubiquitin-specific-processing protease 28 UBP28_HUMAN USP 28 USP28 USP28 protein |
Images
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Fig1:
Western blot analysis of USP28 on different lysates with Rabbit anti-USP28 antibody (ER1803-52) at 1/5,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 3: HaCaT (Human immortalized keratinocyte) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-52, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 122 kDa Observed band size: 140 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.