| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 122 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human USP28 aa 550-700. |
| Positive control: | K562, A549, Daudi, MG-63. |
| Subcellular location: | Nucleus, nucleoplasm. |
| Recommended Dilutions:
WB FC |
1:200-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q96RU2 Human |
| Alternative names: | Deubiquitinating enzyme 28 KIAA1515 Ubiquitin carboxyl terminal hydrolase 28 Ubiquitin carboxyl-terminal hydrolase 28 ubiquitin carboxyl-terminal hydrolase 28 variant 1 Ubiquitin specific peptidase 28 Ubiquitin specific processing protease 28 Ubiquitin specific protease 28 Ubiquitin thioesterase 28 Ubiquitin thiolesterase 28 Ubiquitin-specific-processing protease 28 UBP28_HUMAN USP 28 USP28 USP28 protein |
|
Fig1:
Western blot analysis of USP28 on different cell lysates using anti-USP28 antibody at 1/500 dilution. Positive control: Lane 1: K562 Lane 2: A549 Lane 3: Daudi |
|
Fig2: Flow cytometric analysis of MG-63 cells with USP28 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |