Anti-CDKN2A/p16INK4a antibody
cat.: ER1803-53
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 17 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human CDKN2A/p16INK4a.
Positive control: SiHa, 293T, Hela, human colon cancer tissue, human stomach cancer tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P42771 Human
Alternative names: CCM2 antibody CDK4 inhibitor p16 INK4 antibody CDK4I antibody CDKN2 antibody CDKN2A antibody Cell cycle negative regulator beta antibody CMM2 antibody Cyclin dependent kinase 4 inhibitor A antibody Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody Cyclin Dependent Kinase Inhibitor 2A antibody Cyclin dependent kinase inhibitor 2A isoform 4 antibody Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody Cyclin dependent kinase inhibitor p16 antibody INK4 antibody INK4A antibody MLM antibody MTS1 antibody Multiple tumor suppressor 1 antibody p14 antibody p16 antibody P16INK4 antibody p16INK4a antibody p19 antibody p19Arf antibody TP16 antibody
Images
ER1803-53_1.jpg Fig1: Western blot analysis of CDKN2A/p16INK4a on SiHa cell lysate using anti-CDKN2A/p16INK4a antibody at 1/500 dilution.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: SiHa cell lysate with immunization peptide
ER1803-53_2.jpg Fig2: ICC staining CDKN2A/p16INK4a in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-53_3.jpg Fig3: ICC staining CDKN2A/p16INK4a in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-53_4.jpg Fig4: ICC staining CDKN2A/p16INK4a in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-53_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CDKN2A/p16INK4a antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
ER1803-53_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-CDKN2A/p16INK4a antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
ER1803-53_7.jpg Fig7: Flow cytometric analysis of 293T cells with CDKN2A/p16INK4a antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.