|Product Type:||Rabbit polyclonal IgG, primary antibodies|
|Species reactivity:||Human, Rat, Mouse|
|Applications:||WB, IF-Cell, IHC-P, FC|
|Storage condition:||Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.|
|Storage buffer:||1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.|
|Purification:||Immunogen affinity purified.|
|Molecular weight:||Predicted band size: 31 kDa|
|Immunogen:||Recombinant protein within Human CD40 aa 1-213 / 277.|
|Positive control:||Daudi cell lysates, SiHa, rat spleen tissue, human tonsil tissue.|
|Subcellular location:||Plasma membrane. Secreted.|
|Uniprot #:||SwissProt: P25942 Human | P27512 Mouse
Entrez Gene: 171369 Rat
|Alternative names:||AI326936 B cell associated molecule CD40 B cell surface antigen CD40 B cell-associated molecule B-cell surface antigen CD40 Bp50 CD 40 CD40 CD40 antigen (TNF receptor superfamily member 5) CD40 antigen CD40 molecule CD40 molecule, TNF receptor superfamily member 5 CD40 protein CD40 type II isoform CD40L receptor CDw40 GP39 HIGM1 IGM IMD3 MGC9013 Nerve growth factor receptor related B lymphocyte activation molecule OTTHUMP00000031699 OTTHUMP00000031700 p50 T-BAM TBAM TNF receptor superfamily member 5 TNFRSF5 TNR5_HUMAN TRAP Tumor necrosis factor receptor superfamily , member 5 Tumor necrosis factor receptor superfamily member 5 Tumor necrosis factor receptor superfamily member 5 precursor Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a|
Western blot analysis of CD40 on Daudi cell lysates with Rabbit anti-CD40 antibody (ER1803-54) at 1/2,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 40 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-54) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
|Fig2: ICC staining CD40 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.|
|Fig3: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD40 antibody. Counter stained with hematoxylin.|
|Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD40 antibody. Counter stained with hematoxylin.|
|Fig5: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.|