USP14 Rabbit Polyclonal Antibody
cat.: ER1803-55
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human USP14 aa 226-408 / 494. |
| Positive control: | Daudi cell lysates, SiHa. |
| Subcellular location: | Plasma membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P54578 Human |
| Alternative names: | Deubiquitinating enzyme 14 TGT tRNA guanine transglycosylase 60 kD subunit tRNA guanine transglycosylase Ubiquitin carboxyl terminal hydrolase 14 Ubiquitin carboxyl-terminal hydrolase 14 Ubiquitin specific peptidase 14 Ubiquitin specific processing protease 14 Ubiquitin specific protease 14 Ubiquitin thiolesterase 14 Ubiquitin-specific-processing protease 14 UBP14_HUMAN USP 14 USP14 |
Images
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Fig1:
Western blot analysis of USP14 on different lysates with Rabbit anti-USP14 antibody (ER1803-55) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si USP14 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of USP14 on Daudi cell lysate using anti-USP14 antibody at 1/500 dilution. |
|
Fig3: Flow cytometric analysis of SiHa cells with USP14 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody;black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.