HEC1 Rabbit Polyclonal Antibody
cat.: ER1803-56
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HEC1 aa 300-505 / 642. |
| Positive control: | 293T cell lysates, A431 cell lysates, HeLa cell lysates. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: O14777 Human |
| Alternative names: | from Entrez Gene ; HEC Highly expressed in cancer Highly expressed in cancer protein Highly expressed in cancer rich in leucine heptad repeats HsHec1 hsNDC80 Kinetochore associated 2 Kinetochore associated protein 2 Kinetochore protein Hec1 Kinetochore protein NDC80 homolog Kinetochore-associated protein 2 KNTC2 ndc80 NDC80 homolog kinetochore complex component NDC80 kinetochore complex component homolog NDC80, S. cerevisiae, homolog of NDC80_HUMAN Retinoblastoma associated protein HEC Retinoblastoma-associated protein HEC TID3 |
Images
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Fig1:
Western blot analysis of HEC1 on different lysates with Rabbit anti-HEC1 antibody (ER1803-56) at 1/5,000 dilution. Lane 1: 293T (Human embryonic kidney cell) cell lysate Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-56, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 73.9 kDa Observed band size: 74 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.