ER1803-56

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 74 kDa

Isotype:

IgG

Immunogen:

Recombinant protein within Human HEC1 aa 300-505 / 642.

Positive control:

SiHa cell lysates, HL-60 cell lysates, MG-63.

Subcellular location:

Nucleus.

Recommended Dilutions:
WB
FC

1:500
1:50-1:100

Uniprot #:

SwissProt: O14777 Human

Alternative names:

from Entrez Gene ; HEC Highly expressed in cancer Highly expressed in cancer protein Highly expressed in cancer rich in leucine heptad repeats HsHec1 hsNDC80 Kinetochore associated 2 Kinetochore associated protein 2 Kinetochore protein Hec1 Kinetochore protein NDC80 homolog Kinetochore-associated protein 2 KNTC2 ndc80 NDC80 homolog kinetochore complex component NDC80 kinetochore complex component homolog NDC80, S. cerevisiae, homolog of NDC80_HUMAN Retinoblastoma associated protein HEC Retinoblastoma-associated protein HEC TID3

	Fig1: Western blot analysis of HEC1 on SiHa (1,2) and HL-60 (3,4) cell lysates using anti-HEC1 antibody at 1/500 dilution. Positive control: Lane 1, 3: Anti-HEC1 antibody Lane 2, 4: Anti-HEC1 antibody, with immunization Recombinant protein
	Fig2: Flow cytometric analysis of MG-63 cells with HEC1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.