MCU Rabbit Polyclonal Antibody
cat.: ER1803-57
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MCU aa 51-225 / 351. |
| Positive control: | A549 cell lysate, HL-60 cell lysate, mouse spleen tissue lysates, mouse brain tissue lysates, HT-29, rat brain tissue, human colon tissue, mouse testis tissue, A549. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:500-1:1,000 1:50-1:200 1:50 1:1,000 |
| Uniprot #: | SwissProt: Q8NE86 Human | Q3UMR5 Mouse |
| Alternative names: | C109A_HUMAN C10orf42 Calcium uniporter protein mitochondrial Ccdc109a Coiled-coil domain-containing protein 109A HsMCU Mitochondrial Calcium Uniporter |
Images
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Fig1:
Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution. Positive control: Lane 1: A549 cell lysate Lane 3: mouse spleen tissue lysate Lane 2: HL-60 cell lysate Lane 4: mouse brain tissue lysate |
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Fig2:
Immunocytochemistry analysis of HT-29 cells labeling MCU with Rabbit anti-MCU antibody (ER1803-57) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCU antibody (ER1803-57) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L was used as the secondary antibody. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MCU antibody (ER1803-57) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-57) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MCU antibody (ER1803-57) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-57) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-MCU antibody (ER1803-57) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-57) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Flow cytometric analysis of A549 cells labeling MCU. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-57, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.