MCU Rabbit Polyclonal Antibody
cat.: ER1803-57
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: Predicted band size 40 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human MCU aa 51-225 / 351.
Positive control: A549, HL-60, mouse spleen tissue lysates, mouse brain tissue lysates, A431, HT-29, rat cerebellum tissue, human lung cancer tissue, mouse brain tissue, human colon tissue, human esophagus tissue.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50 -1:200
1:50-1:100
Uniprot #: SwissProt: Q8NE86 Human | Q3UMR5 Mouse
Alternative names: C109A_HUMAN C10orf42 Calcium uniporter protein mitochondrial Ccdc109a Coiled-coil domain-containing protein 109A HsMCU Mitochondrial Calcium Uniporter
Images
ER1803-57_1.jpg Fig1: Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 3: mouse spleen tissue
Lane 2: HL-60
Lane 4: mouse brain tissue
ER1803-57_2.jpg Fig2: ICC staining MCU in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-57_3.jpg Fig3: ICC staining MCU in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-57_4.jpg Fig4: ICC staining MCU in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-57_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-MCU antibody. Counter stained with hematoxylin.
ER1803-57_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MCU antibody. Counter stained with hematoxylin.
ER1803-57_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MCU antibody. Counter stained with hematoxylin.
ER1803-57_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MCU antibody. Counter stained with hematoxylin.
ER1803-57_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MCU antibody. Counter stained with hematoxylin.
ER1803-57_10.jpg Fig10: Flow cytometric analysis of A549 cells with MCU antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.