| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Connexin 32 aa 230-265. |
| Positive control: | PC-3M cell lysate, A431 cell lysate, Human skin tissue lysate, SKOV-3. |
| Subcellular location: | Cell membrane, Cell junction, gap junction. |
| Recommended Dilutions:
WB FC |
1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P08034 Human | P28230 Mouse |
| Alternative names: | Charcot Marie Tooth neuropathy X linked CMTX 1 CMTX CMTX1 Connexin-32 Connexin32 CX 32 Cx32 CXB1_HUMAN GAP junction 28 kDa liver protein Gap junction beta 1 protein Gap junction beta-1 protein Gap junction protein beta 1 32kD Gap junction protein beta 1 GJB 1 GJB1 |
|
Fig1:
Western blot analysis of Connexin-32 on different lysates using anti-Connexin-32 antibody at 1/500 dilution. Positive control: Lane 1: PC-3M cell lysate Lane 2: A431 cell lysate Lane 3: Human skin tissue lysate |
|
Fig2: Flow cytometric analysis of SKOV-3 cells with Connexin-32 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |