Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 32 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within mouse Connexin-32 aa 230-265. |
Positive control: | PC-3M cell lysate, A431 cell lysate, Human skin tissue lysate, SKOV-3. |
Subcellular location: | Cell membrane, Cell junction, gap junction. |
Recommended Dilutions:
WB FC |
1:500 1:50-1:100 |
Uniprot #: | SwissProt: P08034 Human | P28230 Mouse |
Alternative names: | Charcot Marie Tooth neuropathy X linked CMTX 1 CMTX CMTX1 Connexin-32 Connexin32 CX 32 Cx32 CXB1_HUMAN GAP junction 28 kDa liver protein Gap junction beta 1 protein Gap junction beta-1 protein Gap junction protein beta 1 32kD Gap junction protein beta 1 GJB 1 GJB1 |
Fig1:
Western blot analysis of Connexin-32 on different lysates using anti-Connexin-32 antibody at 1/500 dilution. Positive control: Lane 1: PC-3M cell lysate Lane 2: A431 cell lysate Lane 3: Human skin tissue lysate |
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Fig2: Flow cytometric analysis of SKOV-3 cells with Connexin-32 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |