alpha Actinin Rabbit Polyclonal Antibody
cat.: ER1803-60
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 103 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human alpha Actinin aa 388-619 / 892.
Positive control: A431, rat colon tissue lysates, mouse colon tissue lysates, MG-63, SiHa, human colon cancer tissue, human kidney tissue, mouse colon tissue.
Subcellular location: Plasma membrane. Cytoskeleton.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:100
1:50 -1:200
1:50-1:100
Uniprot #: SwissProt: P12814 Human | Q7TPR4 Mouse | Q9Z1P2 Rat
Alternative names: actinin 1 smooth muscle Actinin alpha 1 actinin, alpha 1 ACTN 1 Actn1 ACTN1_HUMAN Alpha Actinin 1 Alpha actinin cytoskeletal isoform Alpha-actinin cytoskeletal isoform Alpha-actinin-1 BDPLT15 F actin cross linking protein F-actin cross-linking protein FLJ40884 FLJ54432 Non muscle alpha actinin 1 Non-muscle alpha-actinin-1
Images
ER1803-60_1.jpg Fig1: Western blot analysis of alpha Actinin on different lysates using anti-alpha Actinin antibody at 1/500 dilution.
Positive control:
Lane 1: A431
Lane 2: Rat colon tissue
Lane 3: Mouse colon tissue
ER1803-60_2.jpg Fig2: ICC staining alpha Actinin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-60_3.jpg Fig3: ICC staining alpha Actinin in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-60_4.jpg Fig4: ICC staining alpha Actinin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-60_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.
ER1803-60_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.
ER1803-60_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.
ER1803-60_8.jpg Fig8: Flow cytometric analysis of MG-63 cells with alpha Actinin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.