alpha Actinin Rabbit Polyclonal Antibody
cat.: ER1803-60
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 103 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human alpha Actinin aa 388-619 / 892. |
| Positive control: | A431 cell lysate, Rat colon tissue lysate, mouse colon tissue lysates, human breast cancer tissue, rat colon tissue, SH-SY5Y, SiHa. |
| Subcellular location: | Cell junction, Cell membrane, Cell projection, Cytoplasm, Plasma membrane. Cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000 1:200 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P12814 Human | Q7TPR4 Mouse | Q9Z1P2 Rat |
| Alternative names: | actinin 1 smooth muscle Actinin alpha 1 actinin, alpha 1 ACTN 1 Actn1 ACTN1_HUMAN Alpha Actinin 1 Alpha actinin cytoskeletal isoform Alpha-actinin cytoskeletal isoform Alpha-actinin-1 BDPLT15 F actin cross linking protein F-actin cross-linking protein FLJ40884 FLJ54432 Non muscle alpha actinin 1 Non-muscle alpha-actinin-1 |
Images
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Fig1:
Western blot analysis of alpha Actinin on different lysates with Rabbit anti-alpha Actinin antibody (ER1803-60) at 1/5,000 dilution. Lane 1: A431 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lane 4: COS-1 cell lysate Lane 5: Mouse heart tissue lysate Lane 6: Rat colon tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-60) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-alpha Actinin antibody (ER1803-60) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-alpha Actinin antibody (ER1803-60) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of SH-SY5Y cells labeling alpha Actinin with Rabbit anti-alpha Actinin antibody (ER1803-60) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Actinin antibody (ER1803-60) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunocytochemistry analysis of Siha cells labeling alpha Actinin with Rabbit anti-alpha Actinin antibody (ER1803-60) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Actinin antibody (ER1803-60) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
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Fig6:
Flow cytometric analysis of SH-SY5Y cells labeling alpha Actinin. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-60, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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