SCNN1G Rabbit Polyclonal Antibody
cat.: ER1803-61
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 74 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SCNN1G aa 160-342 / 649.
Positive control: A431cell lysates, 293T, A431, rat kidney tissue, mouse brain tissue.
Subcellular location: Plasma membrane.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P51170 Human | Q9WU39 Mouse | P37091 Rat
Alternative names: Amiloride sensitive epithelial sodium channel gamma subunit Amiloride sensitive sodium channel subunit gamma Amiloride-sensitive sodium channel subunit gamma BESC3 ENaC gamma subunit ENaCG ENaCgamma Epithelial Na(+) channel subunit gamma Epithelial Na+ channel subunit gamma Gamma ENaC Gamma NaCH Gamma-ENaC Gamma-NaCH Nonvoltage gated sodium channel 1 subunit gamma Nonvoltage-gated sodium channel 1 subunit gamma PHA 1 PHA1 SCNEG SCNN 1G SCNN1G SCNNG_HUMAN Sodium channel epithelial 1 gamma subunit Sodium channel non voltage gated 1 gamma subunit Sodium channel nonvoltage gated 1 gamma
Images
ER1803-61_1.jpg Fig1: Western blot analysis of SCNN1G on A431cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1803-61, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ER1803-61_2.jpg Fig2: ICC staining of SCNN1G in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1803-61, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1803-61_3.jpg Fig3: ICC staining of SCNN1G in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1803-61, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1803-61_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SCNN1G antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1803-61_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SCNN1G antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1803-61_6.jpg Fig6: Flow cytometric analysis of SCNN1G was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1803-61, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.