CASK Rabbit Polyclonal Antibody
cat.: ER1803-62
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CASK aa 300-500. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, SiHa cell lysate, A549 cell lysate, SiHa,HT-29. |
| Subcellular location: | Cell membrane, Nucleus, Cytoplasm, Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O14936 Human | Q62915 Rat |
| Alternative names: | CAGH39 Caki Calcium/calmodulin dependent serine protein kinase Calcium/calmodulin dependent serine protein kinase (MAGUK family) Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase Calcium/calmodulin-dependent serine protein kinase CAMGUK CAMGUK protein CAMGUK, drosophila, homolog of casK CMG CSKP_HUMAN DXPri1 DXRib1 FGS4 FLJ22219 FLJ31914 hCASK LIN 2 Lin 2 homolog LIN2 Lin2 homolog MICPCH MRXSNA Pals3 Peripheral plasma membrane protein CASK Protein lin-2 homolog TNRC8 Trinucleotide repeat containing 8 Vertebtate LIN2 homolog |
Images
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Fig1:
Western blot analysis of CASK on different lysates with Rabbit anti-CASK antibody (ER1803-62) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-62) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CASK on different cell lysate using anti-CASK antibody at 1/500 dilution. Positive control: Lane 1: SiHa cell lysate Lane 2: A549 cell lysate |
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Fig3: ICC staining CASK in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Flow cytometric analysis of HT-29 cells with CASK antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.