CASK Rabbit Polyclonal Antibody
cat.: ER1803-62
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 105/59 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CASK aa 300-500.
Positive control: SiHa, A549, HT-29, rat brain tissue, human appendix tissue, human prostate cancer tissue, human placenta tissue.
Subcellular location: Plasma membrane. Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:100-1:200
1:50-1:100
Uniprot #: SwissProt: O14936 Human | Q62915 Rat
Alternative names: CAGH39 Caki Calcium/calmodulin dependent serine protein kinase Calcium/calmodulin dependent serine protein kinase (MAGUK family) Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase Calcium/calmodulin-dependent serine protein kinase CAMGUK CAMGUK protein CAMGUK, drosophila, homolog of casK CMG CSKP_HUMAN DXPri1 DXRib1 FGS4 FLJ22219 FLJ31914 hCASK LIN 2 Lin 2 homolog LIN2 Lin2 homolog MICPCH MRXSNA Pals3 Peripheral plasma membrane protein CASK Protein lin-2 homolog TNRC8 Trinucleotide repeat containing 8 Vertebtate LIN2 homolog
Images
ER1803-62_1.jpg Fig1: Western blot analysis of CASK on different cell lysate using anti-CASK antibody at 1/500 dilution.
Positive control:
Lane 1: SiHa
Lane 2: A549
ER1803-62_2.jpg Fig2: ICC staining CASK in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-62_3.jpg Fig3: ICC staining CASK in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-62_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CASK antibody. Counter stained with hematoxylin.
ER1803-62_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CASK antibody. Counter stained with hematoxylin.
ER1803-62_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-CASK antibody. Counter stained with hematoxylin.
ER1803-62_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CASK antibody. Counter stained with hematoxylin.
ER1803-62_8.jpg Fig8: Flow cytometric analysis of HT-29 cells with CASK antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.