SDHB Rabbit Polyclonal Antibody
cat.: ER1803-63
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 32 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SDHB aa 100-280.
Positive control: Rat liver tissue, human liver tissue, mouse liver tissue, 293T, HT-29, Hela, rat heart tissue, human kidney tissue, human skin tissue, mouse colon tissue.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P21912 Human | Q9CQA3 Mouse | P21913 Rat
Alternative names: CWS2 DHSB_HUMAN FLJ92337 Ip Iron sulfur subunit Iron sulfur subunit of complex II Iron-sulfur subunit of complex II mitochondrial PGL 4 PGL4 SDH 1 SDH SDH1 SDH2 SDH2, homolog of SdhB SDHIP Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial Succinate dehydrogenase [ubiquinone] iron sulfur subunit Succinate dehydrogenase [ubiquinone] iron-sulfur subunit succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Succinate Dehydrogenase 1 Iron Sulfur Subunit Succinate Dehydrogenase 2, S. cerevisiae, homolog of Succinate dehydrogenase complex iron sulfur subunit B Succinate dehydrogenase complex subunit B iron sulfur Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein succinate dehydrogenase complex, subunit B, iron sulfur (Ip) Succinate dehydrogenase iron sulfur protein
Images
ER1803-63_1.jpg Fig1: All lanes: Western blot analysis of SDHB with anti-SDHB antibody (ER1803-63) at 1:1,000 dilution.
Lane 1: Wild-type Hela whole cell lysate.
Lane 2: SDHB knockout Hela whole cell lysate.

ER1803-63 was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout samples were tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (ER1803-63, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ER1803-63_2.jpg Fig2: Western blot analysis of SDHB on different cell lysate using anti-SDHB antibody at 1/1,000 dilution.
Positive control:
Lane 1: human liver
Lane 2: mouse liver
Lane 3: rat liver
Lane 4: rat liver, preincubated with the immunization protein.
ER1803-63_3.jpg Fig3: ICC staining SDHB in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-63_4.jpg Fig4: ICC staining SDHB in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1803-63_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-SDHB antibody. Counter stained with hematoxylin.
ER1803-63_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin.
ER1803-63_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SDHB antibody. Counter stained with hematoxylin.
ER1803-63_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin.
ER1803-63_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHB antibody. Counter stained with hematoxylin.
ER1803-63_10.jpg Fig10: Flow cytometric analysis of 293T cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.