SDHB Rabbit Polyclonal Antibody
cat.: ER1803-63
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SDHB aa 100-280. |
| Positive control: | Wild-type Hela whole cell lysate, Rat liver tissue, human liver tissue, mouse liver tissue, 293T, HT-29, Hela, rat heart tissue, human liver tissue, human kidney tissue, human skin tissue, mouse colon tissue. |
| Subcellular location: | Membrane, Mitochondrion, Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P21912 Human | Q9CQA3 Mouse | P21913 Rat |
| Alternative names: | CWS2 DHSB_HUMAN FLJ92337 Ip Iron sulfur subunit Iron sulfur subunit of complex II Iron-sulfur subunit of complex II mitochondrial PGL 4 PGL4 SDH 1 SDH SDH1 SDH2 SDH2, homolog of SdhB SDHIP Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial Succinate dehydrogenase [ubiquinone] iron sulfur subunit Succinate dehydrogenase [ubiquinone] iron-sulfur subunit succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Succinate Dehydrogenase 1 Iron Sulfur Subunit Succinate Dehydrogenase 2, S. cerevisiae, homolog of Succinate dehydrogenase complex iron sulfur subunit B Succinate dehydrogenase complex subunit B iron sulfur Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein succinate dehydrogenase complex, subunit B, iron sulfur (Ip) Succinate dehydrogenase iron sulfur protein |
Images
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Fig1:
All lanes: Western blot analysis of SDHB with anti-SDHB antibody (ER1803-63) at 1/1,000 dilution. Lane 1: Wild-type Hela whole cell lysate. Lane 2: SDHB knockout Hela whole cell lysate. ER1803-63 was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout sample was tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (ER1803-63, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig2:
Western blot analysis of SDHB on different lysates using anti-SDHB antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lane 4: Rat liver tissue lysate, preincubated with the immunization protein. |
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Fig3: ICC staining SDHB in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining SDHB in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-SDHB antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SDHB antibody. Counter stained with hematoxylin. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SDHB antibody (ER1803-63) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-63) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHB antibody. Counter stained with hematoxylin. |
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Fig10: Flow cytometric analysis of 293T cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.