NrCAM Rabbit Polyclonal Antibody
cat.: ER1803-64
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 144 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NRCAM aa 723-903 / 1,304. |
| Positive control: | SH-SY5Y cell lysate, A549, SH-SY5Y, SKOV-3, rat brain tissue, mouse brain tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:200 1:50 -1:200 |
| Uniprot #: | SwissProt: Q92823 Human | Q810U4 Mouse | P97686 Rat |
| Alternative names: | Bravo hBravo Neuronal cell adhesion molecule Neuronal surface protein Bravo Ng CAM related Ng-CAM-related NgCAM related cell adhesion molecule NgCAM-related cell adhesion molecule Nr CAM Nr-CAM Nrcam NRCAM_HUMAN |
Images
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Fig1: ICC staining NRCAM in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining NRCAM in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining NRCAM in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin. |
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Fig6:
Western blot analysis of NrCAM on different lysates with Rabbit anti-NrCAM antibody (ER1803-64) at 1/1,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate(20 µg/Lane) Lane 2: Mouse brain tissue lysate(30 µg/Lane) Exposure time: 60 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-64, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 144 kDa Observed band size: 144 kDa |
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Fig7:
Western blot analysis of NrCAM on different lysates with Rabbit anti-NrCAM antibody (ER1803-64) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate(30 µg/Lane) Exposure time: 60 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-64, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 144 kDa Observed band size: 144 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.