ITPR2 Rabbit Polyclonal Antibody
cat.: ER1803-65
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | Dot Blot, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 308 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ITPR2 aa 1100-1450. |
| Positive control: | HepG2, HT-29, SiHa, rat kidney tissue, human colon tissue, human breast tissue, mouse liver tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
Dot Blot IF-Cell IHC-P FC |
1:2,000-1:5,000 1:50-1:100 1:50 -1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q14571 Human | Q9Z329 Mouse | P29995 Rat |
| Alternative names: | 4 5-trisphosphate receptor Inositol 1 4 5 trisphosphate receptor type 2 Inositol 1,4,5-trisphosphate receptor type 2 Inositol 145 trisphosphate receptor type 2 InsP3 R2 InsP3R2 IP3 R2 IP3 receptor IP3 receptor isoform 2 IP3R 2 IP3R2 ITPR 2 Itpr2 ITPR2_HUMAN Type 2 inositol 1 4 5 trisphosphate receptor Type 2 inositol 1 Type 2 inositol 145 trisphosphate receptor Type 2 InsP3 receptor |
Images
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Fig1: Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500. |
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Fig2: ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of HepG2 cells with ITPR2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.