GTSF1 Rabbit Polyclonal Antibody
cat.: ER1803-68
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Zebrafish |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within zebrafish GTSF1 aa 1-182 / 182. |
| Positive control: | Zebrafish lysate, Zebrafish. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: K4FBI1 Zebrafish |
| Alternative names: | Cue110 Fam112b Family with sequence similarity 112 member B FLJ32942 Gametocyte-specific factor 1 GTSF1 GTSF1_HUMAN Protein FAM112B |
Images
|
Fig1: Western blot analysis of GTSF1 on zebrafish lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/200 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunofluorescence staining of paraffin-embedded Zebrafish using anti-GTSF1 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (ER1803-68) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-GTSF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-68) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.