Annexin A1 Rabbit Polyclonal Antibody
cat.: ER1803-70
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Annexin A1 aa 150-310.
Positive control: A431 cell lysate, rat uterus tissue lysate, A431, A549, SiHa, rat lung tissue, human esophagus tissue, human placenta tissue, mouse prostate tissue.
Subcellular location: Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane, Nucleus, Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:1,000-1:2,000
1:50-1:200
1:200
Uniprot #: SwissProt: P04083 Human | P10107 Mouse | P07150 Rat
Alternative names: Annexin 1 Annexin A1 Annexin I (lipocortin I) Annexin I Annexin-1 AnnexinA1 AnnexinI ANX 1 ANX A1 ANX1 ANXA 1 ANXA1 ANXA1 protein ANXA1_HUMAN Calpactin 2 Calpactin II Calpactin-2 CalpactinII Chromobindin 9 Chromobindin-9 Chromobindin9 HGNC:533 Lipocortin 1 Lipocortin I Lipocortin1 LipocortinI LPC 1 LPC1 p35 Phospholipase A2 inhibitory protein
Images
ER1803-70_1.jpg Fig1: Western blot analysis of Annexin A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: Rat uterus tissue lysate
ER1803-70_2.jpg Fig2: ICC staining Annexin A1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Annexin A1 polyclonal antibody at a dilution of 1/200 for at least 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1803-70_3.jpg Fig3: ICC staining Annexin A1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Annexin A1 polyclonal antibody at a dilution of 1/50 for at least 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1803-70_4.jpg Fig4: ICC staining Annexin A1 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Annexin A1 polyclonal antibody at a dilution of 1/100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1803-70_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Annexin A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-70) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-70_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Annexin A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-70) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-70_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Annexin A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-70) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-70_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Annexin A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-70) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.