CD13 Rabbit Polyclonal Antibody
cat.: ER1803-74
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD13 aa 273-448 / 967. |
| Positive control: | Rat kidney tissue lysates, human liver tissue lysates, A549, HT-29, human kidney tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:100-1:400 1:20,000 1:50-1:100 |
| Uniprot #: | SwissProt: P15144 Human | P97449 Mouse | P15684 Rat |
| Alternative names: | Alanyl (membrane) aminopeptidase Alanyl aminopeptidase Aminopeptidase M Aminopeptidase N AMPN_HUMAN ANPEP AP M AP N AP-M AP-N APN CD 13 CD13 CD13 antigen gp150 hAPN LAP 1 LAP1 Microsomal aminopeptidase Myeloid plasma membrane glycoprotein CD13 p150 PEPN |
Images
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Fig1: Western blot analysis of CD13 on rat kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CD13 on human liver tissue lysate with Rabbit anti-CD13 antibody (ER1803-74) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 140 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-74) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining CD13 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with CD13 polyclonal antibody at a dilution of 1:500 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining CD13 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with CD13 polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD13 antibody (ER1803-74) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-74) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of CD13 was done on A549 cells. The cells were fixed, permeabilized and stained with CD13 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.