Mesothelin Rabbit Polyclonal Antibody
cat.: ER1803-76
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Mesothelin aa 220-410. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, NIH:OVCAR8 (Human ovarian adenocarcinoma cell) cell lysate, SiHa (Human cervical cancersSquamous cell) cell lysate, NCI-H226 (Human pleural mesothelioma cell) cell lysate, human tonsil tissue, human lung cancer tissue. |
| Subcellular location: | Cell membrane, Golgi apparatus, Membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:50 1:50-1:200 |
| Uniprot #: | SwissProt: Q13421 Human |
| Alternative names: | CAK 1 CAK1 CAK1 antigen cleaved form Megakaryocyte potentiating factor Mesothelin Mesothelin isoform 1 precursor MPF Msln MSLN_HUMAN Pre pro megakaryocyte potentiating factor Pre-pro-megakaryocyte-potentiating factor SMR SMRP Soluble MPF mesothelin related protein |
Images
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Fig1:
Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (ER1803-76) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: NIH:OVCAR8 (Human ovarian adenocarcinoma cell) cell lysate Lane 3: PC-3 (Human prostate cancer cell) cell lysate Lane 4: SiHa (Human cervical cancersSquamous cell) cell lysate Lane 5: A549 (Human lung adenocarcinoma cell) cell lysate Lane 6: NCI-H226 (Human pleural mesothelioma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 16 seconds; ECL: K1801 PC-3 is a negative control (PMID: 27760836). A549 is a low expression (PMID: 25118887). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-76, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 69 kDa Observed band size: 45 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-76) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-76) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.