Anti-Mesothelin antibody
cat.: ER1803-76
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Protein affinity purified.
Molecular weight: 45 kDa, cleavage form
Isotype: IgG
Immunogen: Recombinant protein within Human Mesothelin aa 220-410.
Positive control: Human tonsil tissue, human lung cancer tissue, SiHa, Hela.
Subcellular location: Cell membrane, Golgi apparatus, Membrane, Secreted.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:2,000
1:50
1:50-1:200
Uniprot #: SwissProt: Q13421 Human
Alternative names: CAK 1 antibody CAK1 antibody CAK1 antigen antibody cleaved form antibody Megakaryocyte potentiating factor antibody Mesothelin antibody Mesothelin isoform 1 precursor antibody MPF antibody Msln antibody MSLN_HUMAN antibody Pre pro megakaryocyte potentiating factor antibody Pre-pro-megakaryocyte-potentiating factor antibody SMR antibody SMRP antibody Soluble MPF mesothelin related protein antibody
Images
ER1803-76_1.jpg Fig1: Western blot analysis of Mesothelin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: Hela cell lysate
ER1803-76_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-76) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-76_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-76) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.