CD99 Rabbit Polyclonal Antibody
cat.: ER1803-81
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 25 kDa (predicted molecular weight: 19 kDa)
Isotype: IgG
Immunogen: Recombinant protein within Human CD99 aa 1-185 / 185.
Positive control: SiHa, HepG2, rat kidney tissue, human esophagus tissue, human pancreas tissue, mouse testis tissue, 293T.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P14209 Human | Q8VCN6 Mouse
Alternative names: 12E7 Antigen identified by monoclonal 12E7, Y homolog Antigen identified by monoclonal antibodies 12E7, F21 and O13 CD99 CD99 antigen CD99 molecule CD99_HUMAN Cell surface antigen 12E7 Cell surface antigen HBA 71 Cell surface antigen O13 E2 antigen HBA71 MIC 2X MIC 2Y MIC2 (monoclonal 12E7) MIC2 MIC2X MIC2Y MSK5X Protein MIC2 Surface antigen MIC2 T cell surface glycoprotein E2 T-cell surface glycoprotein E2
Images
ER1803-81_1.jpg Fig1: Western blot analysis of CD99 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: HepG2 cell lysate
ER1803-81_2.jpg Fig2: ICC staining CD99 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1803-81) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1803-81_3.jpg Fig3: ICC staining CD99 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1803-81) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1803-81_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CD99 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-81) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-81_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-CD99 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-81) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-81_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD99 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-81) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-81_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CD99 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-81) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-81_8.jpg Fig8: Flow cytometric analysis of CD99 was done on 293T cells. The cells were fixed, permeabilized and stained with CD99 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.