Galectin 3 Rabbit Polyclonal Antibody
cat.: ER1803-82
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 26 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Galectin 3 aa 1-180.
Positive control: SiHa, mouse colon tissue, A431, LOVO, rat lung tissue, human colon cancer tissue, mouse testis tissue.
Subcellular location: Cytoplasm, Nucleus, Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P17931 Human | P16110 Mouse | P08699 Rat
Alternative names: 35 kDa lectin Carbohydrate binding protein 35 Carbohydrate-binding protein 35 CBP 35 CBP35 Gal-3 GAL3 Galactose-specific lectin 3 Galactoside-binding protein GALBP Galectin 3 internal gene,included Galectin-3 Galectin3 GALIG GBP IgE binding protein IgE-binding protein L 31 L 34 L-31 L-34 galactoside-binding lectin L31 Laminin-binding protein Lectin L-29 Lectin, galactose binding, soluble 3 LEG3_HUMAN LGALS2 LGALS3 MAC 2 antigen Mac-2 Mac-2 antigen MAC2 Macrophage galactose-specific lectin MGC105387
Images
ER1803-82_1.jpg Fig1: Western blot analysis of Galectin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: Mouse colon tissue lysate
ER1803-82_2.jpg Fig2: ICC staining Galectin 3 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1803-82) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1803-82_3.jpg Fig3: ICC staining Galectin 3 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1803-82) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1803-82_4.jpg Fig4: ICC staining Galectin 3 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1803-82) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1803-82_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Galectin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-82) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-82_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Galectin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-82) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-82_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Galectin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-82) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-82_8.jpg Fig8: Flow cytometric analysis of Galectin 3 was done on SiHa cells. The cells were fixed, permeabilized and stained with Galectin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.