IL-22 Rabbit Polyclonal Antibody
cat.: ER1803-85
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | ELISA, WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 20 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IL-22 aa 34-179. |
| Positive control: | Rat kidney tissue, human tonsil tissue, human breast cancer tissue, mouse colon tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA WB IHC-P |
1:5000-1:10,000 1:500 1:50-1:200 |
| Uniprot #: | SwissProt: Q9GZX6 Human | Q9JJY9 Mouse Entrez Gene: 500836 Rat |
| Alternative names: | Cytokine Zcyto18 IL 10 related T cell derived inducible factor IL 21 IL 22 IL D110 IL TIF IL-10-related T-cell-derived-inducible factor IL-22 IL-TIF IL21 Il22 IL22_HUMAN ILD110 ILTIF Interleukin 10 related T cell derived inducible factor interleukin 21 Interleukin 22 Interleukin-22 MGC79382 MGC79384 TIFa TIFIL 23 TIFIL23 UNQ3099/PRO10096 zcyto18 |
Images
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Fig1:
Western blot analysis of IL-22 on IL22 recombinant protein with Rabbit anti-IL-22 antibody (ER1803-85) at 1/20,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-85, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 20 kDa Observed band size: 18 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-IL-22 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-85) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-22 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-85) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breasr cancer tissue using anti-IL-22 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-85) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-22 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-85) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.