MSH2 Rabbit Polyclonal Antibody
cat.: ER1803-86
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 104 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MSH2 aa 1-150. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P43246 Human |
| Alternative names: | BAT26 COCA 1 COCA1 DNA mismatch repair protein Msh2 FCC 1 FCC1 hMSH2 HNPCC 1 HNPCC HNPCC1 LCFS2 MSH 2 Msh2 MSH2_HUMAN MutS homolog 2 MutS homolog 2 colon cancer nonpolyposis type 1 MutS protein homolog 2 |
Images
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Fig1:
Western blot analysis of MSH2 on different lysates with Rabbit anti-MSH2 antibody (ER1803-86) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane) Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate (15 µg/Lane) Lane 3: LNCaP (Human prostate cancer cell) cell lysate (15 µg/Lane) Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate (15 µg/Lane) Lane 5: Mouse testis tissue lysate (30 µg/Lane) Lane 6: Rat testis tissue lysate (30 µg/Lane) Exposure time: 6 seconds; ECL: K1801 LNCaP is a negative control (PMID: 11025778). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-86, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 104 kDa Observed band size: 104 kDa |
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Fig2:
All lanes: Western blot analysis of MSH2 with anti-MSH2 antibody (ER1803-86) at 1:1,000 dilution. Lane 1: Wild-type SCC7 whole cell lysate. Lane 2: MSH2 knockout SCC7 whole cell lysate. ER1803-86 was shown to specifically react with MSH2 in wild-type SCC7 cells. No band was observed when MSH2 knockout samples were tested. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MSH2 antibody (ER1803-86, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.