MSH2 Rabbit Polyclonal Antibody
cat.: ER1803-86
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 104 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MSH2 aa 1-150. |
| Positive control: | K562 cell lysates, Wild-type SCC7 whole cell lysates. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: P43246 Human |
| Alternative names: | BAT26 COCA 1 COCA1 DNA mismatch repair protein Msh2 FCC 1 FCC1 hMSH2 HNPCC 1 HNPCC HNPCC1 LCFS2 MSH 2 Msh2 MSH2_HUMAN MutS homolog 2 MutS homolog 2 colon cancer nonpolyposis type 1 MutS protein homolog 2 |
Images
|
Fig1:
All lanes: Western blot analysis of MSH2 with anti-MSH2 antibody (ER1803-86) at 1:1,000 dilution. Lane 1: Wild-type SCC7 whole cell lysate. Lane 2: MSH2 knockout SCC7 whole cell lysate. ER1803-86 was shown to specifically react with MSH2 in wild-type SCC7 cells. No band was observed when MSH2 knockout samples were tested. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MSH2 antibody (ER1803-86, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of MSH2 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.