G-protein coupled receptor 30 Rabbit Polyclonal Antibody
cat.: ER1803-90
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human G-protein coupled receptor 30 aa 236-375 / 375. |
| Positive control: | LoVo, SH-SY5Y. |
| Subcellular location: | Mitochondrion,Nucleus,Endosome,Golgi apparatus,Endoplasmic reticulum,Cytoskeleton. |
| Recommended Dilutions:
IF-Cell FC |
1:100 1:1,000 |
| Uniprot #: | SwissProt: Q99527 Human |
| Alternative names: | CEPR Chemoattractant receptor-like 2 Chemokine receptor-like 2 CMKRL2 Constitutively expressed peptide like receptor DRY12 FEG 1 FEG-1 Flow-induced endothelial G protein-coupled receptor Flow-induced endothelial G-protein coupled receptor 1 G protein-coupled receptor 30 G-protein coupled estrogen receptor 1 G-protein coupled receptor 30 GPCR-BR Gper GPER_HUMAN GPER1 GPR30 IL8-related receptor DRY12 Lergu LERGU2 leucine rich protein in GPR30 3'UTR LYGPR Lymphocyte-derived G-protein coupled receptor Membrane estrogen receptor mER MGC99678 |
Images
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Fig1:
Immunocytochemistry analysis of LoVo cells labeling G-protein coupled receptor 30 with Rabbit anti-G-protein coupled receptor 30 antibody (ER1803-90) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-G-protein coupled receptor 30 antibody (ER1803-90) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling G-protein coupled receptor 30 with Rabbit anti-G-protein coupled receptor 30 antibody (ER1803-90) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-G-protein coupled receptor 30 antibody (ER1803-90) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Flow cytometric analysis of SH-SY5Y cells labeling G-protein coupled receptor 30. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-90, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.