G-protein coupled receptor 30 Rabbit Polyclonal Antibody
cat.: ER1803-90
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 42 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human G-protein coupled receptor 30 aa 236-375 / 375.
Positive control: Lovo cell lysates, LoVo, SH-SY5Y, rat uterus tissue, human liver cancer tissue, human appendix tissue, human placenta tissue, mouse kidney tissue.
Subcellular location: Mitochondrion,Nucleus,Endosome,Golgi apparatus,Endoplasmic reticulum,Cytoskeleton.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1,000
1:100
1:50-1:200
1:1,000
Uniprot #: SwissProt: Q99527 Human | Q8BMP4 Mouse | O08878 Rat
Alternative names: CEPR Chemoattractant receptor-like 2 Chemokine receptor-like 2 CMKRL2 Constitutively expressed peptide like receptor DRY12 FEG 1 FEG-1 Flow-induced endothelial G protein-coupled receptor Flow-induced endothelial G-protein coupled receptor 1 G protein-coupled receptor 30 G-protein coupled estrogen receptor 1 G-protein coupled receptor 30 GPCR-BR Gper GPER_HUMAN GPER1 GPR30 IL8-related receptor DRY12 Lergu LERGU2 leucine rich protein in GPR30 3'UTR LYGPR Lymphocyte-derived G-protein coupled receptor Membrane estrogen receptor mER MGC99678
Images
ER1803-90_1.jpg Fig1: Western blot analysis of G-protein coupled receptor 30 on Lovo cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1803-90_2.jpg Fig2: Immunocytochemistry analysis of LoVo cells labeling G-protein coupled receptor 30 with Rabbit anti-G-protein coupled receptor 30 antibody (ER1803-90) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-G-protein coupled receptor 30 antibody (ER1803-90) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ER1803-90_3.jpg Fig3: Immunocytochemistry analysis of SH-SY5Y cells labeling G-protein coupled receptor 30 with Rabbit anti-G-protein coupled receptor 30 antibody (ER1803-90) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-G-protein coupled receptor 30 antibody (ER1803-90) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ER1803-90_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-G-protein coupled receptor 30 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-90) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-90_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-G-protein coupled receptor 30 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-90) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-90_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-G-protein coupled receptor 30 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-90) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-90_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-G-protein coupled receptor 30 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-90) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-90_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-G-protein coupled receptor 30 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-90) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-90_9.jpg Fig9: Flow cytometric analysis of SH-SY5Y cells labeling G-protein coupled receptor 30.

Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-90, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.