| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TRPA1 aa 505-755 / 1,119. |
| Positive control: | L-929 (Mouse fibroblast) cell lysates. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: O75762 Human | Q8BLA8 Mouse | Q6RI86 Rat |
| Alternative names: | ANKTM 1 ANKTM1 Ankyrin-like with transmembrane domains protein 1 Transformation sensitive protein p120 Transformation-sensitive protein p120 Transient receptor potential cation channel subfamily A member 1 TRPA 1 Trpa1 TRPA1_HUMAN |
|
Fig1:
Western blot analysis of TRPA1 on L-929 (Mouse fibroblast) cell lysate with Rabbit anti-TRPA1 antibody (ER1803-91) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-91, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 127 kDa Observed band size: 150 kDa |