RYR3 Rabbit Polyclonal Antibody
cat.: ER1803-92
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | Dot Blot, IHC-P, IF-Cell, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 552 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RYR3 aa 1-140 / 4,870. |
| Positive control: | Lovo, rat brain tissue, human kidney tissue, human placenta tissue, mouse brain tissue. |
| Subcellular location: | Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum. |
| Recommended Dilutions:
ELISA Dot Blot IF-Cell IHC-P |
1:5,000-1:10,000 1:2000-1:20,000 1:100 1:50-1:200 |
| Uniprot #: | SwissProt: Q15413 Human | Q14AQ9 Mouse Entrez Gene: 170546 Rat |
| Alternative names: | Brain ryanodine receptor-calcium release channel Brain-type ryanodine receptor Type 3 ryanodine receptor Ryanodine receptor 3 Brain ryanodine receptor calcium release channel Brain type ryanodine receptor Cardiac muscle ryanodine receptor calcium release channel Cardiac muscle type ryanodine receptor CCO Central core disease of muscle HBRR MHS Ryanodine receptor 3 Ryanodine receptor type1 RYDR RYR 3 RYR RYR3 Sarcoplasmic reticulum calcium release channel Skeletal muscle calcium release channel Skeletal muscle ryanodine receptor Skeletal muscle type ryanodine receptor Skeletal muscle-type ryanodine receptor SKRR VTSIP |
Images
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Fig1: Dot blot analysis of RYR3 on RYR3 recombinant protein. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining RYR3 in Lovo cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1803-92) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RYR3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-92) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RYR3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-92) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-RYR3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-92) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-RYR3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-92) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.