Cytokeratin 17 Rabbit Polyclonal Antibody
cat.: ER1803-94
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Cytokeratin 17 aa 100-300.
Positive control: A431, SiHa, rat prostate tissue, human tonsil tissue, human lung cancer tissue, human breast tissue, mouse skin tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:1,000-1:5000
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: Q04695 Human | Q9QWL7 Mouse | Q6IFU8 Rat
Alternative names: 39.1 CK 17 CK17 CK-17 Cytokeratin-17 K17 K1C17_HUMAN Keratin 17 keratin 17 epitope S1 keratin 17 epitope S2 keratin 17 epitope S4 Keratin 17, type I Keratin Keratin type I cytoskeletal 17 keratin, type i cytoskeletal 17 [version 1] Keratin-17 KRT17 PC PC2 PCHC1 type I cytoskeletal 17
Images
ER1803-94_1.jpg Fig1: Western blot analysis of Cytokeratin 17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: SiHa cell lysate
ER1803-94_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat prostate tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-94) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-94_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-94) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-94_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-94) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-94_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-94) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-94_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-94) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1803-94_7.jpg Fig7: Flow cytometric analysis of Cytokeratin 17 was done on Hela cells. The cells were fixed, permeabilized and stained with MMP9 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.