BOB1 Rabbit Polyclonal Antibody
cat.: ER1803-97
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BOB1 aa 10-180. |
| Positive control: | Daudi (Human Burkitt's lymphoma cell) cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, Ramos (Human Burkitt's lymphoma cell) cell lysate, Daudi. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:5,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q16633 Human | Q64693 Mouse Entrez Gene: 690528 Rat |
| Alternative names: | B cell Oct binding protein 1 B cell specific coactivator OBF 1 B cell specific coactivator OBF1 B-cell-specific coactivator OBF-1 BOB 1 BOB-1 OBF 1 OBF1 OBF1_HUMAN OCA B OCA-B OCAB OCT binding factor 1 OCT-binding factor 1 POU class 2 associating factor 1 POU domain class 2 associating factor 1 POU domain class 2-associating factor 1 Pou2af1 |
Images
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Fig1:
Western blot analysis of BOB1 on different lysates with Rabbit anti-BOB1 antibody (ER1803-97) at 1/5,000 dilution. Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 2: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 3: Ramos (Human Burkitt's lymphoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 8 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1803-97, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 27 kDa Observed band size: 36 kDa |
|
Fig2: Flow cytometric analysis of BOB1 was done on Daudi cells. The cells were fixed, permeabilized and stained with BOB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.