| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 17 aa 117-141. |
| Positive control: | SiHa. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q04695 Human | Q9QWL7 Mouse | Q6IFU8 Rat |
| Alternative names: | 39.1 CK 17 CK17 CK-17 Cytokeratin-17 K17 K1C17_HUMAN Keratin 17 keratin 17 epitope S1 keratin 17 epitope S2 keratin 17 epitope S4 Keratin 17, type I Keratin Keratin type I cytoskeletal 17 keratin, type i cytoskeletal 17 [version 1] Keratin-17 KRT17 PC PC2 PCHC1 type I cytoskeletal 17 |
|
Fig1:
Western blot analysis of Cytokeratin 17 on different lysates with Rabbit anti-Cytokeratin 17 antibody (ER1901-01) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 3: MCF7 (Human breast cancer cell) cell lysate(negative) Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1901-01, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 48 kDa |