Brd4 Rabbit Polyclonal Antibody
cat.: ER1901-02
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 152 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Brd4 aa 1-120.
Positive control: A549, SiHa, mouse testis tissue, human kidney tissue, rat brain tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  IF-Cell
  IHC-P
1:50-1:200
1:50-1:500
Uniprot #: SwissProt: O60885 Human | Q9ESU6 Mouse
Entrez Gene: 362844 Rat
Alternative names: Brd4 BRD4-NUT FUSION BRD4-NUT fusion oncoprotein BRD4_HUMAN Bromodomain containing 4 bromodomain containing protein 4 Bromodomain-containing protein 4 CAP chromosome associated protein HUNK1 HUNKI MCAP Mitotic chromosome-associated protein Protein HUNK1
Images
ER1901-02_1.jpg Fig1: ICC staining of Brd4 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-02, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1901-02_2.jpg Fig2: ICC staining of Brd4 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-02, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1901-02_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Brd4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-02, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-02_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Brd4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-02, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-02_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Brd4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-02, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.