Anti-delta 1 Catenin/CAS antibody
cat.: ER1901-07
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 108 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal Human delta 1 Catenin/CAS
Positive control: Human skin tissue, mouse stomach tissue, SiHa, human breast cancer tissue, human kidney tissue, mouse testis tissue.
Subcellular location: Cell membrane, Cytoplasm, Membrane, Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:2000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: O60716 Human | P30999 Mouse
Alternative names: Cadherin associated Src substrate antibody Cadherin-associated Src substrate antibody CAS antibody Catenin (cadherin associated protein) delta 1 antibody Catenin delta 1 antibody Catenin delta antibody Catenin delta-1 antibody CTND1_HUMAN antibody CTNND 1 antibody CTNND antibody CTNND1 antibody delta 1 Catenin antibody KIAA0384 antibody p120 antibody P120 CAS antibody p120 catenin antibody P120 CTN antibody p120(cas) antibody p120(ctn) antibody P120CAS antibody P120CTN antibody
Images
ER1901-07_1.jpg Fig1: Western blot analysis of delta 1 Catenin/CAS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-07, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human skin tissue lysate
Lane 2: Mouse stomach tissue lysate
ER1901-07_2.jpg Fig2: ICC staining of delta 1 Catenin/CAS in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-07, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-07_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-delta 1 Catenin/CAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-07, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-07_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-delta 1 Catenin/CAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-07, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-07_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-delta 1 Catenin/CAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-07, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.