| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rice |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Rice Os01g0847700 aa 262-311 / 311. |
| Positive control: | Rice stem. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:500-1:1000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q0JHR5 Rice |
|
Fig1: Western blot analysis of Rice Os01g0847700 protein on rice stem lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: IF staining of Rice Os01g0847700 protein in rice stem tissue(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rice stem tissue using anti-Rice Os01g0847700 protein antibody. Counter stained with hematoxylin. |