SSRP1 Rabbit Polyclonal Antibody
cat.: ER1901-13
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 81 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SSRP1 aa 192-375 / 709.
Positive control: Daudi cell lysate, Hela cell lysate, 293 cell lysate, Jurkat cell lysate, rat testis tissue lysate, mouse testis tissue lysate, rat testis tissue, human skin tissue, human breast cancer tissue, mouse colon tissue, SH-SY5Y.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q08945 Human | Q08943 Mouse | Q04931 Rat
Alternative names: Chromatin-specific transcription elongation factor 80 kDa subunit Cisplatin DNA SSRP Facilitates chromatin remodeling 80 kDa subunit Facilitates chromatin transcription complex 80 kDa subunit Facilitates chromatin transcription complex subunit SSRP1 FACT 80 kDa subunit FACT complex subunit SSRP1 FACT80 FACTp80 hSSRP1 Recombination signal sequence recognition protein 1 Recombination signal sequence recognition protein SSRP 1 SSRP1 SSRP1_HUMAN Structure specific recognition protein 1 Structure-specific recognition protein 1 T160
Images
ER1901-13_1.jpg Fig1: Western blot analysis of SSRP1 on different lysates with Rabbit anti-SSRP1 antibody (ER1901-13) at 1/500 dilution.

Lane 1: Daudi cell lysate (10 µg/Lane)
Lane 1: Hela cell lysate (10 µg/Lane)
Lane 1: 293 cell lysate (10 µg/Lane)
Lane 1: Jurkat cell lysate (10 µg/Lane)
Lane 2: Rat testis tissue lysate (20 µg/Lane)
Lane 2: Mouse testis tissue lysate (20 µg/Lane)

Predicted band size: 81 kDa
Observed band size: 81 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-13) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER1901-13_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-SSRP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-13_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SSRP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-13_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-SSRP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-13_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SSRP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-13_6.jpg Fig6: Flow cytometric analysis of SSRP1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-13, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.