NPY2R / Y2 receptor Rabbit Polyclonal Antibody
cat.: ER1901-14
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse NPY2R / Y2 receptor aa 327-376 / 381. |
| Positive control: | HUVEC cell lysates, MCF7. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P49146 Human |
| Alternative names: | Neuropeptide Y receptor type 2 Neuropeptide Y receptor Y2 NPY-Y2 receptor NPY2-R NPY2R NPY2R_HUMAN Y2 receptor |
Images
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Fig1:
Western blot analysis of NPY2R / Y2 receptor on HUVEC cell lysates with Rabbit anti-NPY2R / Y2 receptor antibody (ER1901-14) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-14) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of MCF7 cells labeling NPY2R / Y2 receptor. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1901-14, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.