Anti-FSH beta antibody
cat.: ER1901-19
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL
Purification: Peptide affinity purified.
Molecular weight: 15 kDa (Predicted band size)
Isotype: IgG
Immunogen: Synthetic peptide within human FSH beta aa 40-80.
Positive control: SH-SY5Y cell, rat pituitary tissue, human pituitary tissue, human liver tissue, mouse pituitary tissue, SH-SY5Y.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:2,000
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: P01225 Human | Q60687 Mouse | P18427 Rat
Alternative names: Follicle Stimulating Hormone antibody Follicle stimulating hormone beta polypeptide antibody Follicle stimulating hormone beta subunit antibody Follitropin beta chain antibody Follitropin subunit beta antibody FSH-B antibody FSH-beta antibody FSHB antibody FSHbeta antibody hide
Images
ER1901-19_1.jpg Fig1: Western blot analysis of FSH beta on SH-SY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-19, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-19_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat pituitary tissue using anti-FSH beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-19_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human pituitary tissue using anti-FSH beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-19_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-FSH beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-19_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue using anti-FSH beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-19_6.jpg Fig6: Flow cytometric analysis of FSH beta was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-19, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.