FSH beta Rabbit Polyclonal Antibody
cat.: ER1901-19
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FSH beta aa 37-86 / 129. |
| Positive control: | Mouse pituitary tissue lysates, mouse pituitary tissue, rat pituitary tissue, SH-SY5Y. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:1,000-1:4,000 1:50-1:100 |
| Uniprot #: | SwissProt: P01225 Human | Q60687 Mouse | P18427 Rat |
| Alternative names: | Follicle Stimulating Hormone Follicle stimulating hormone beta polypeptide Follicle stimulating hormone beta subunit Follitropin beta chain Follitropin subunit beta FSH-B FSH-beta FSHB FSHbeta hide |
Images
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Fig1:
Western blot analysis of FSH beta on mouse pituitary tissue lysates with Rabbit anti-FSH beta antibody (ER1901-19) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 15 kDa Observed band size: 25 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-19) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-FSH beta antibody (ER1901-19) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-19) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-FSH beta antibody (ER1901-19) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-19) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of FSH beta was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-19, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.