GATA3 Rabbit Polyclonal Antibody
cat.: ER1901-20
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 48 kDa (Predicted band size)
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human GATA3.
Positive control: MCF-7, rat bladder tissue.
Subcellular location: Nucleus.
Recommended Dilutions:

Uniprot #: SwissProt: P23771 Human
Alternative names: GATA 3 GATA binding factor 3 GATA binding protein 3 GATA-binding factor 3 Gata3 GATA3_HUMAN HDR HDRS MGC2346 MGC5199 MGC5445 Trans acting T cell specific transcription factor GATA 3 Trans-acting T-cell-specific transcription factor GATA-3
ER1901-20_1.jpg Fig1: Western blot analysis of GATA3 on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Specific bands were detected for GATA3 full length (FL) at approximately 52 kDa and the splice form (SF) at approximately 39 kDa (as indicated).
ER1901-20_2.jpg Fig2: ICC staining of GATA3 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1901-20_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-GATA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-20, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-20_4.jpg Fig4: Flow cytometric analysis of GATA3 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-20, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).