Anti-TRPML3 antibody
cat.: ER1901-21
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL
Purification: Peptide affinity purified.
Molecular weight: 64 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse TRPML3 aa 400-553.
Positive control: 293 cell, F9.
Subcellular location: Early endosome membran, late endosome membrane, stereocilium membrane, autophagosome membrane.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:50
50-1:200
1:50-1:100
Uniprot #: SwissProt: Q8TDD5 Human | Q8R4F0 Mouse
Alternative names: MCOLN 3 MCOLN3 FLJ11006 FLJ36629 MCLN3_HUMAN Mcoln3 MGC71509 Mucolipin 3 Mucolipin-3 TRP ML3 TRPML3
Images
ER1901-21_1.jpg Fig1: Western blot analysis of TRPML3 on 293 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-21_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-TRPML3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-21, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-21_3.jpg Fig3: Flow cytometric analysis of TRPML3 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-21, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.