TRPML3 Rabbit Polyclonal Antibody
cat.: ER1901-21
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TRPML3 aa 504-553 / 553. |
| Positive control: | A549 cell lysate, HL-60 cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat brain tissue lysate, rat kidney tissue lysate, human liver carcinoma tissue, F9. |
| Subcellular location: | Early endosome membran, late endosome membrane, stereocilium membrane, autophagosome membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50 50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q8TDD5 Human | Q8R4F0 Mouse Entrez Gene: 308022 Rat |
| Alternative names: | MCOLN 3 MCOLN3 FLJ11006 FLJ36629 MCLN3_HUMAN Mcoln3 MGC71509 Mucolipin 3 Mucolipin-3 TRP ML3 TRPML3 |
Images
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Fig1:
Western blot analysis of TRPML3 on different lysates with Rabbit anti-TRPML3 antibody (ER1901-21) at 1/2,000 dilution. Lane 1: A549 cell lysate (10 µg/Lane) Lane 2: HL-60 cell lysate (10 µg/Lane) Lane 3: HEK-293 cell lysate (10 µg/Lane) Lane 4: Mouse brain tissue lysate (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Lane 7: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-TRPML3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-21, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of TRPML3 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-21, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.