KV1.1 Rabbit Polyclonal Antibody
cat.: ER1901-23
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse KV11 aa 175-224 / 495. |
| Positive control: | Rat brain tissue lysate, rat spinal cord tissue, mouse brain tissue, A549. |
| Subcellular location: | Endoplasmic reticulum. Plasma membrane. Other locations. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q09470 Human | P16388 Mouse | P10499 Rat |
| Alternative names: | AEMK EA1 Episodic ataxia with myokymia HBK1 HUK1 Kca1 1 Kcna1 KCNA1_HUMAN Kcpvd KV1.1 MBK1 mceph MGC124402 MGC126782 MGC138385 MK1 MK1, mouse, homolog of KV1.1 Potassium channel protein 1 Potassium voltage gated channel shaker related subfamily member 1 Potassium voltage gated channel subfamily A member 1 Potassium voltage gated channel, shaker related subfamily, member 1 (episodic ataxia with myokymia) Potassium voltage-gated channel subfamily A member 1 RBK1 RCK1 Shak Shaker related subfamily member 1 Voltage gated potassium channel subunit Kv1.1 Voltage-gated K(+) channel HuKI Voltage-gated potassium channel HBK1 Voltage-gated potassium channel subunit Kv1.1 |
Images
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Fig1: Western blot analysis of KV1.1 on rat brain tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-KV1.1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-23, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KV1.1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-23, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of KV1.1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-23, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). FC M |
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