FoxP1 Rabbit Polyclonal Antibody
cat.: ER1901-26
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 75 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal of human FoxP1.
Positive control: HepG2 cell lysate, rat spleen tissue lysate, rat brain tissue, human colon tissue, human breast tissue, human tonsil tissue, MCF-7.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:2,000
1:200-1:1,000
1:50-1:100
Uniprot #: SwissProt: Q9H334 Human | Q498D1 Rat
Alternative names: 12CC4 FLJ23741 Fork head related protein like B Forkhead box P1 Forkhead box protein P1 FOX P1 FOXP 1 foxp1 FOXP1_HUMAN Glutamine rich factor 1 hFKH1B HSPC215 MGC12942 MGC88572 MGC99551 QRF 1 QRF1
Images
ER1901-26_1.jpg Fig1: Western blot analysis of FoxP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: rat spleen tissue lysate
ER1901-26_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-FoxP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-26_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-FoxP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-26_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-FoxP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-26_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FoxP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-26, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-26_6.jpg Fig6: Flow cytometric analysis of FoxP1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-26, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.