DOG1 Rabbit Polyclonal Antibody
cat.: ER1901-30
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 114/97 kDa (Predicted band size)
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human DOG1.
Positive control: PC-3M cell lysates, A549, HepG2, human liver carcinoma tissue, human seminal pouch tissue.
Subcellular location: Cell membrane, cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q5XXA6 Human
Alternative names: ANO 1 ANO1 ANO1_HUMAN Anoctamin 1 Anoctamin 1 calcium activated chloride channel Anoctamin-1 Anoctamin1 Ca2+ activated Cl- channel Calcium Activated Chloride Channel Discovered on gastrointestinal stromal tumors protein 1 DOG 1 DOG1 FLJ10261 Membrane protein Oral cancer overexpressed 2 Oral cancer overexpressed protein 2 ORAOV 2 ORAOV2 TAOS 2 TAOS2 TMEM 16A TMEM16A Transmembrane protein 16A (eight membrane spanning domains) Transmembrane protein 16A Tumor amplified and overexpressed sequence 2 Tumor-amplified and overexpressed sequence 2
Images
ER1901-30_1.jpg Fig1: Western blot analysis of DOG1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-30_2.jpg Fig2: ICC staining of DOG1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1901-30_3.jpg Fig3: ICC staining of DOG1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1901-30_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-DOG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-30_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human seminal pouch tissue using anti-DOG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-30_6.jpg Fig6: Flow cytometric analysis of DOG1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-30, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.