ER1901-30

TMEM16A Rabbit Polyclonal Antibody

cat.: ER1901-30

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	114/97 kDa (Predicted band size)
Isotype:	IgG
Immunogen:	Synthetic peptide within C-terminal human TMEM16A.
Positive control:	A549 cell lysates, PC-3M cell lysate, A549, HepG2, human liver carcinoma tissue, human seminal pouch tissue.
Subcellular location:	Cell membrane, cytoplasm.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:500-1:1000 1:50-1:200 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: Q5XXA6 Human
Alternative names:	ANO 1 ANO1 ANO1_HUMAN Anoctamin 1 Anoctamin 1 calcium activated chloride channel Anoctamin-1 Anoctamin1 Ca2+ activated Cl- channel Calcium Activated Chloride Channel Discovered on gastrointestinal stromal tumors protein 1 DOG 1 DOG1 FLJ10261 Membrane protein Oral cancer overexpressed 2 Oral cancer overexpressed protein 2 ORAOV 2 ORAOV2 TAOS 2 TAOS2 TMEM 16A TMEM16A Transmembrane protein 16A (eight membrane spanning domains) Transmembrane protein 16A Tumor amplified and overexpressed sequence 2 Tumor-amplified and overexpressed sequence 2

Images

	Fig1: Western blot analysis of TMEM16A on different lysates with Rabbit anti-TMEM16A antibody (ER1901-30) at 1/1,000 dilution. Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate Lane 2: PC-3M (Human prostate cancer cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1901-30, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 97 kDa Observed band size: 97 kDa
	Fig2: ICC staining of TMEM16A in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig3: ICC staining of TMEM16A in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

	Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-TMEM16A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1901-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human seminal pouch tissue using anti-TMEM16A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1901-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Flow cytometric analysis of TMEM16A was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-30, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.