CD68 Rabbit Polyclonal Antibody
cat.: ER1901-32
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Cow |
| Applications: | WB, IHC-P, ICC, IF, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD68 aa 25-74 / 354. |
| Subcellular location: | Cell membrane, endosome membrane, lysosome membrane. |
| Recommended Dilutions:
WB IHC-P ICC IF ELISA |
1:500-1:2,000 1:100-1:500 1:100 : 1:100-1:500 1:5,000-10,000 |
| Uniprot #: | SwissProt: P34810 Human | P31996 Mouse |
| Alternative names: | CD 68 CD68 CD68 antigen CD68 molecule CD68_HUMAN DKFZp686M18236 gp11 Gp110 LAMP4 Macrophage antigen CD68 (microsialin) MACROPHAGE ANTIGEN CD68 Macrosialin SCARD1 Scavenger receptor class D member 1 |
Images
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Fig1: This image was generously provided by Amanda Brown, Ph.D. from Johns Hopkins University School of Medicine. Paraffin embedded human brain tissue labeled with Rabbit Anti-CD68 Polyclonal Antibody, Unconjugated (ER1901-32) at 1:50 followed by conjugation to the secondary antibody and Permanent Fast Red Quanto staining |
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Fig2:
Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated(ER1901-32) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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Fig3:
Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 2: Lymph node (Rat) Cell Lysate at 30 ug Primary: Anti-CD68 (ER1901-32) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution Predicted band size: 110/185-200 kD Observed band size: 110 kD |
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Fig4:
Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug Lane 2: A549 (Human) Cell Lysate at 30 ug Lane 3: Jurkat (Human) Cell Lysate at 30 ug Lane 4: Huvec (Human) Cell Lysate at 30 ug Primary: Anti-CD68 (ER1901-32) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution Predicted band size: 110/185-200 kD Observed band size: 110 kD |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.