AKT1/2/3 Rabbit Polyclonal Antibody
cat.: ER1901-33
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 56 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human AKT1 aa 211-383 / 479.
Positive control: MCF7 cell lysate, Jurkat cell lysate, C6 cell lysate, rat heart tissue lysate, A549, EA.hy926, human thyroid tissue, human kidney tissue, mouse brain tissue.
Subcellular location: Nucleus, Cytoplasm, Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P31749 Human | P31751 Human | Q9Y243 Human | P31750 Mouse | Q60823 Mouse | Q9WUA6 Mouse | P47196 Rat | P47197 Rat | Q63484 Rat
Alternative names: AKT AKT1 AKT1 kinase AKT1m AKT2 AKT2 kinase Akt3 AKT3_HUMAN CAKT CWS6 DKFZp434N0250 HIHGHH kinase Akt1 MGC99656 MPPH Murine thymoma viral (v-akt) homolog 2 PKB ALPHA PKB PKB beta PKB gamma PKB-GAMMA PKB/Akt PKBALPHA PKBB PKBBETA PKBG PKBGAMMA PRKBA PRKBB PRKBG Protein kinase Akt 2 Protein kinase Akt-3 Protein kinase B alpha Protein kinase B Protein kinase B beta Protein kinase B gamma Proto oncogene c Akt RAC ALPHA RAC alpha serine/threonine protein kinase RAC RAC BETA RAC beta serine/threonine protein kinase RAC PK alpha RAC PK beta rac protein kinase alpha rac protein kinase beta RAC-gamma RAC-gamma serine/threonine-protein kinase RAC-PK-gamma RACALPHA RACalpha serine/threonine kinase RACBETA RACgamma RACgamma serine/threonine protein kinase RACPKgamma serine threonine protein kinase STK-2 STK2 thymoma viral proto oncogene 1 thymoma viral proto oncogene V akt murine......
Images
ER1901-33_1.jpg Fig1: Western blot analysis of AKT1/2/3 on different lysates with Rabbit anti-AKT1/2/3 antibody (ER1901-33) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: C6 cell lysate
Lane 4: Rat heart tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 18 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-33) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1901-33_2.jpg Fig2: ICC staining of AKT1/2/3 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1901-33_3.jpg Fig3: ICC staining of AKT1/2/3 in EA.hy926 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1901-33_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-AKT1/2/3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-33, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-33_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AKT1/2/3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-33, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-33_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AKT1/2/3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-33, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-33_7.jpg Fig7: Flow cytometric analysis of AKT1/2/3 was done on EA.hy926 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-33, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.