KCNAB1 Rabbit Polyclonal Antibody
cat.: ER1901-34
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46.5 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse KCNAB1 aa 36-85 / 401. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, rat cerebellum tissue lysate, EA.hy926, SH-SY5Y. |
| Subcellular location: | Cell membrane, cytoplasm, membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:50-1:100 1:100-1:500 |
| Uniprot #: | SwissProt: Q14722 Human | P63143 Mouse | P63144 Rat |
| Alternative names: | hKvb3 hKvBeta3 K(+) channel subunit beta-1 KCAB1_HUMAN KCNA1B KCNAB1 Kv-beta-1 Kvb1.3 Voltage-gated potassium channel beta-1 subunit Voltage-gated potassium channel subunit beta-1 |
Images
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Fig1:
Western blot analysis of KCNAB1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysates Lane 2: Rat brain tissue lysates Lane 3: Rat cerebellum tissue lysates |
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Fig2: ICC staining of KCNAB1 in EA.hy926 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Flow cytometric analysis of KCNAB1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-34, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.