ATG4A Rabbit Polyclonal Antibody
cat.: ER1901-38
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ATG4A aa 87-314 / 398. |
| Positive control: | Human placenta tissue, mouse testis tissue, rat bone marrow tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1000 1:50-1:200 |
| Uniprot #: | SwissProt: Q8WYN0 Human | Q8C9S8 Mouse |
| Alternative names: | AI627006 APG4 autophagy 4 homolog A Apg4a ATG4 autophagy related 4 homolog A (S. cerevisiae) ATG4 autophagy related 4 homolog A ATG4A ATG4A_HUMAN Atg4al AUT like 2 cysteine endopeptidase AUT-like 2 cysteine endopeptidase Autl2 Autophagin 2 Autophagin-2 Autophagy related 4A cysteine peptidase Autophagy related cysteine endopeptidase 2 Autophagy related protein 4 homolog A Autophagy-related cysteine endopeptidase 2 Autophagy-related protein 4 homolog A AV169859 Cysteine protease ATG4A hAPG4A MGC107179 |
Images
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Fig1: Western blot analysis of ATG4A on rat bone marrow tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ATG4A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-38, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATG4A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-38, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.