Syntrophin alpha 1 Rabbit Polyclonal Antibody
cat.: ER1901-40
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse Syntrophin alpha 1 aa 174-223 / 503.
Positive control: Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, SW620, SiHa.
Subcellular location: Sarcolemma, cytoskeleton, cell junction.
Recommended Dilutions:
  WB
  ICC
  FC

1:500-1:2,000
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: Q13424 Human | Q61234 Mouse
Alternative names: 59 kDa dystrophin-associated protein A1 acidic component 1 Alpha-1-syntrophin Pro-TGF-alpha cytoplasmic domain-interacting protein 1 SNT1 SNT2 SNT3 Snta1 SNTA1_HUMAN SNTB1 SNTB2 Syntrophin alpha 1 Syntrophin beta 1 Syntrophin beta 2 Syntrophin-1 TACIP1
Images
ER1901-40_1.jpg Fig1: Western blot analysis of Syntrophin alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat skeletal muscle tissue lysate
ER1901-40_2.jpg Fig2: ICC staining of Syntrophin alpha 1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1901-40_3.jpg Fig3: Flow cytometric analysis of Syntrophin alpha 1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.