Anti-KCNB1 antibody
cat.: ER1901-41
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 96 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal rat KCNB1.
Positive control: Rat cerebellum tissue lysates, rat brain tissue, mouse brain tissue, SH-SY5Y.
Subcellular location: Cell membrane, postsynaptic cell membrane, lateral cell membrane, sarcolemma.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q14721 Human | Q03717 Mouse | P15387 Rat
Alternative names: Delayed rectifier potassium channel 1 antibody Delayed rectifier potassium channel Kv2.1 antibody DRK 1 antibody DRK1 antibody h DRK1 K(+) channel antibody h-DRK1 antibody hDRK 1 antibody hDRK1 antibody KCB 1 antibody KCB1 antibody KCNB1 antibody KCNB1_HUMAN antibody KV2.1 antibody Potassium channel protein DRK1 antibody Potassium voltage gated channel shab related subfamily member 1 antibody Potassium voltage-gated channel subfamily B member 1 antibody Voltage-gated potassium channel subunit Kv2.1 antibody
Images
ER1901-41_1.jpg Fig1: Western blot analysis of KCNB1 on rat cerebellum tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-41_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-KCNB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-41, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-41_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KCNB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-41_4.jpg Fig4: Flow cytometric analysis of KCNB1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-41, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.