Syntrophin alpha 1 Rabbit Polyclonal Antibody
cat.: ER1901-45
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse Syntrophin alpha 1 aa 174-223 / 503.
Positive control: Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, SW620, human kidney tissue, mouse skeletal muscle tissue, SiHa.
Subcellular location: Sarcolemma, cytoskeleton, cell junction.
Recommended Dilutions:
  WB
  IHC-P
  ICC
  FC

1:500-1:2,000
1:50-1:100
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: Q13424 Human | Q61234 Mouse
Alternative names: 59 kDa dystrophin-associated protein A1 acidic component 1 Alpha-1-syntrophin Pro-TGF-alpha cytoplasmic domain-interacting protein 1 SNT1 SNT2 SNT3 Snta1 SNTA1_HUMAN SNTB1 SNTB2 Syntrophin alpha 1 Syntrophin beta 1 Syntrophin beta 2 Syntrophin-1 TACIP1
Images
ER1901-45_1.jpg Fig1: Western blot analysis of Syntrophin alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse skeletal muscle tissue lysate
Lane 2: rat skeletal muscle tissue lysate
ER1901-45_2.jpg Fig2: ICC staining of Syntrophin alpha 1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1901-45_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Syntrophin alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-45_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Syntrophin alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-45_5.jpg Fig5: Flow cytometric analysis of Syntrophin alpha 1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-45, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.