Anti-P2X6 antibody
cat.: ER1901-49
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal rat P2rx6.
Positive control: Jurkat cell lysate, K562 cell lysate, rat skeletal muscle tissue, rat brain tissue, mouse skeletal muscle tissue, mouse heart tissue, SH-SY5Y.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O15547 Human | O54803 Mouse | P51579 Rat
Alternative names: ATP receptor antibody P2RX6 antibody P2RX6_HUMAN antibody P2RXL1 antibody P2X purinoceptor 6 antibody P2X6 antibody P2XM antibody Purinergic receptor antibody Purinergic receptor P2X ligand gated ion channel 6 antibody Purinergic receptor P2X like 1 antibody Purinergic receptor P2X-like 1 antibody Purinoceptor P2X6 antibody Purinoreceptor P2X6 antibody Skeletal muscle expressed P2X antibody
Images
ER1901-49_1.jpg Fig1: Western blot analysis of P2X6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate
ER1901-49_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-P2X6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-49_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P2X6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-49_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-P2X6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-49_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-P2X6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-49_6.jpg Fig6: Flow cytometric analysis of P2X6 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.