Arogenate dehydratase Rabbit Polyclonal Antibody
cat.: ER1901-51
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rice |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rice Arogenate dehydratase aa 28-77 / 364. |
| Positive control: | Rice tissue lysates, rice tissue. |
| Subcellular location: | Chloroplast stroma. |
| Recommended Dilutions:
WB IHC-P |
1:100-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: Q6Z3Y3 Rice | ORYSJ Rice |
| Alternative names: | Os07g0694600 |
Images
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Fig1:
Western blot analysis of Arogenate dehydratase on rice tissue lysate with Rabbit anti-Arogenate dehydratase antibody (ER1901-51) at 1/100 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% BSA in PBS, 1 hour at room temperature Primary antibody: ER1901-51, 1/100 in 5% BSA at room temperature for 2 hours Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/5000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 40 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rice tissue using anti-Arogenate dehydratase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.