P2RX1 Rabbit Polyclonal Antibody
cat.: ER1901-53
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human P2X1 aa 252-301 / 399.
Positive control: Mouse brain tissue lysate, mouse cerebellum tissue lysate, SHG-44, SiHa, SKOV-3, rat brain tissue, human prostate carcinoma tissue, human kidney carcinoma tissue, mouse brain tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:50-1:200
Uniprot #: SwissProt: P51575 Human
Alternative names: ATP receptor H.sapiens mRNA for ATP receptor P2 RX1 P2rx1 P2RX1 protein P2RX1_HUMAN P2X purinoceptor 1 P2X receptor subunit 1 P2X1 P2X1 receptor Purinergic receptor Purinergic receptor P2X ligand gated ion channel 1 Purinergic receptor P2X1
Images
ER1901-53_1.jpg Fig1: Western blot analysis of P2RX1 on different lysates with Rabbit anti-P2RX1 antibody (ER1901-53) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Mouse cerebellum tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 45 kDa
Observed band size: 50 kDa

Exposure time: 1 minute 18 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-53) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1901-53_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P2RX1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-53_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-P2RX1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-53_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using anti-P2RX1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-53_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-P2RX1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.