GABRD Rabbit Polyclonal Antibody
cat.: ER1901-54
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within rat GABRD aa 1-80/449.
Positive control: HT-29 cell lysates, MCF-7.
Subcellular location: Postsynaptic cell membrane, cell membrane.
Recommended Dilutions:
  WB
  FC

1:1,000-1:3,000
1:50-1:100
Uniprot #: SwissProt: O14764 Human | P18506 Mouse
Alternative names: GABA(A) receptor subunit delta Gabrd Gamma aminobutyric acid GABA A receptor delta Gamma aminobutyric acid receptor delta subunit precursor GABA A receptor Gamma-aminobutyric acid receptor subunit delta GBRD_HUMAN MGC45284
Images
ER1901-54_1.jpg Fig1: Western blot analysis of GABRD on HT-29 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-54, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-54_2.jpg Fig2: Flow cytometric analysis of GABRD was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-54, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.