GABRD Rabbit Polyclonal Antibody
cat.: ER1901-54
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat GABRD aa 1-80/449. |
| Positive control: | HT-29 cell lysates, MCF-7. |
| Subcellular location: | Postsynaptic cell membrane, cell membrane. |
| Recommended Dilutions:
WB FC |
1:1,000-1:3,000 1:50-1:100 |
| Uniprot #: | SwissProt: O14764 Human | P18506 Mouse |
| Alternative names: | GABA(A) receptor subunit delta Gabrd Gamma aminobutyric acid GABA A receptor delta Gamma aminobutyric acid receptor delta subunit precursor GABA A receptor Gamma-aminobutyric acid receptor subunit delta GBRD_HUMAN MGC45284 |
Images
|
Fig1: Western blot analysis of GABRD on HT-29 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-54, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of GABRD was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-54, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.