KV2.2 Rabbit Polyclonal Antibody
cat.: ER1901-55
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 103 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat KV22 aa 842-891 / 907. |
| Positive control: | SH-SY5Y cell lysates, rat brain tissue, rat smooth muscle tissue, A431. |
| Subcellular location: | Cell membrane, perikaryon, dendrite. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q92953 Human | Q63099 Rat |
| Alternative names: | delayed rectifier potassium channel protein KCNB2 KCNB2_HUMAN potassium channel Kv2.2 potassium voltage gated channel subfamily B member 2 Potassium voltage-gated channel subfamily B member 2 Voltage-gated potassium channel subunit Kv2.2 |
Images
|
Fig1: Western blot analysis of KV2.2 on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-KV2.2 antibody (ER1901-55) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-55) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-KV2.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Flow cytometric analysis of KV2.2 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-55, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.